An atlas of the clinical microbiology of infectious by Edward J. Bottone

By Edward J. Bottone

content material: v. 1. Bacterial brokers --
v. 2. Viral, fungal, and parasitic agents.

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Additional info for An atlas of the clinical microbiology of infectious diseases. / Volume 1, Bacterial agents

Example text

Coli strains usually produce β­hemolytic colonies.  K.  (Pantoea) agglomerans colonies are yellow­pigmented.  Other associations have included brain abscess, thorn­induced eye and wound injuries, and septic arthritis.  vulgaris are strong urease producers.  Four species comprise the genus: S.  Shigellosis is characterized by watery or bloody diarrhea (dysenteric) with mucoid stools. Shigellae are enteroinvasive and can cause necrosis of the colonic epithelium.  Colonies of Salmonella typhi (typhoid bacillus) may appear green without dark centers Page 53 Figure 181 Salmonella species Giemsa stain of bone marrow aspirate of child with AIDS showing intracellular bacilli in macrophage.

Tuberculosis Figure 87 Mycobacterium tuberculosis Apple­green to yellow fluorescing bacilli in sputum smear stained with rhodamine—auramine.  Granules represent individual colonies and are comprised of tightly interwoven bacilli in cord formation Page 33 Morphology The bacilli are either straight or curved to elongated rods, often occurring singly or banded together (cording) because of their lipid­rich hydrophobic surface.  tuberculosis requires enriched media for growth, as provided by Lowenstein­Jensen (egg­based) and Middlebrook 7H10 and 7H11 (serum­based) media.

Colonies resemble those produced by Gordona species.  tuberculosis complex (M.  Mycobacterium leprae, the causative agent of leprosy, does not grow on bacteriologic media.  Now called a tubercle, the focus becomes surrounded by fibroblasts and its center undergoes casseous necrosis.  A separate acid­fast smear of same specimen revealed acid­fast bacilli, and culture grew M. tuberculosis Figure 87 Mycobacterium tuberculosis Apple­green to yellow fluorescing bacilli in sputum smear stained with rhodamine—auramine.

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